These procedures are specific to microbiology based on the 2012 asm guidelines for bsl 2 teaching labs and are in addition to the general safety guidelines. Cover any open cuts on hands and other exposed skin surfaces andor wear gloves. The fem microbiology action team fem document number 200901 october 7, 2009 revision. Clinical microbiology procedures handbook, isenberg hd ed. Laboratory manual for veterinary microbiology c ertificate c ertified that this is a bonafide record of practical work done in the laboratory for the course of veterinary microbiology vmc unit i during the year.
Laboratory science course in how to appropriately wash hands and when hands should be washed. All the standard procedures used in bsl1 applies in addition to other precautions. This 10th edition represents the collaborative efforts of 22 editors and more than 260 authors from around the world, all experienced researchers and practitioners. Microbiology laboratory includes procedures and test methods, work instructions i.
Microbiology a manual society for general microbiology sgm. Laboratory certification manual for drinking water. Microbiology safety and staff induction manual version 6. Microbiology laboratory safety and basic procedures safety in a microbiology laboratory is important in the prevention of infection that might be. Pdf medical microbiology laboratory manual second edition 2009. The types of laboratory equipment and culture media needed to develop and main tain pure cultures. Microbiology a laboratory manual 12th edition pdf free. The concept of sterility and the procedures necessary for. The purpose of this manual is to provide teachers and technicians with good techniques in practical microbiology to ensure that investigations proceed safely and achieve the required educational aims successfully. Known for its thorough coverage, straightforward procedures, and minimal equipment requirements, the eleventh edition incorporates current safety. The competency of microbiology professionals is obtained through the theoretical knowledge gained in the class and the practical skills and applications that lead to the better understanding and interpretations the subject matter.
Effects research laboratory office of research and development stacy pfaller, ph. Microbiology laboratory safety and basic procedures safety in a microbiology laboratory is important in the prevention of infection that might be caused by the microorganisms being studied. Pdf veterinary microbiology laboratory manual sultan a. Pdf applied microbiology laboratory manual lasinrang. Blood culture manual prepared by qa committee issued by. These procedure and protocol manuals are provided from our microbiology laboratory and are available as an educational reference for other laboratories. The laboratory has sufficient quantities of equipment and supplies to carry out the volume of work. April 2018 4 specimen collection the collection of correct microbiology specimens, taken at the appropriate time is essential in the diagnosis of infection and disease. The microbiology department is situated at the john radcliffe hospital, in the main building. This lab manual was created to support a microbiology.
Oxford university hospitals department of microbiology user manual nhs foundation trust iso 15189. Suite 716 san diego, ca 92110 patricia mcvay, md, chief lab director signature date remarks approved revised signature date remarks. Laboratory gowns are provided for your protection and must be worn inside the laboratory and are not to be worn outside the laboratory for any reason. The table is organized alphabetically by disease or agent for easy. The final portion of this manual involves the identification. In microbiology, these items can include, but are not limited to. Mention the general calculations for the experiment. Only closedtoe shoes are to be worn in the laboratory.
It constantly evolves as more information is added to the continuum of knowledge, and as microbiological techniques are rapidly modified and refined. Specimens must be sent to the laboratory in a timely fashion see section 7 for specimen. The microbiology lab has some additional safety considerations. Face protection goggles, mask, face shield, or other splatter guard must be used for all procedures when such procedures could produce splashes or sprays of infectious or other hazardous materials. An introduction to microbiology, aseptic technique and safety preparation safety guidelines 1 risk assessment 2 good microbiological laboratory practice gmlp 3 spillage management 3 aerosols 3 resources equipment 4 apparatus 5 materials 5 media, sterilisation and disinfection preparation of culture media 6 pouring a plate 6 storage of media 6. After the completion of medical laboratory technology, lab scientists lab technologists produced. Microbiological techniques are different in many ways when compared with other laboratory disciplines.
Region 10 laboratory manchester, wa office of research and development. This 10th edition represents the collaborative efforts of 22 editors and more than 260 authors from around the world, all experienced researchers and practitioners in medical and diagnostic microbiology. Laboratory safety general safety rules and procedures 1. At the beginning and termination of each laboratory session, bench tops are to be wiped with a disinfectant solution. Wear closedtoe shoes that cover the top of the foot, and heel. Basic laboratory procedures in clinical bacteriology j. However, many types of microorganisms are potentially pathogenic. Department of microbiology clinical laboratory manual. Standard procedure used to dispose any contaminated material. Standard safety practices in the microbiology laboratory. Each organization should first identify all of its accreditors and requirements to determine if it is more efficient to have multiple quality manuals, a single overarching quality manual or one quality manual with multiple.
Observe an appropriate disposal procedure for broken glass if present. Medical microbiology manual bluegrass community and technical. Safety goggles or safety glasses with side shields are required for laboratory procedures involving bacterial cultures. The first 6 labs should flow as presented in the manual to allow for students to have a solid foundation in the microbiology concepts. June 2002 overview this microbiology laboratory manual is designed especially for the nonmajors, health science. Revision 21, issued may 1, 2019 laboratory testing inc. Mouth pipetting is strictly forbidden in the microbiology laboratory. The target audience is primarily students preparing for a career in the health sciences, however many of the. The exercises in this laboratory manual are designed to engage students in handon activities that reinforce their understanding of the microbial world. To maintain internal links, we recommend the use of microsoft internet explorer.
In using the microscope always follow the appropriate procedure as described in. This lab manual was created to support a microbiology course for allied health students. Who basic laboratory procedures in clinical bacteriology. The laboratory manual references best practices from the clinical and laboratory standards institute clsi, the foundation for innovative new diagnostics find mgit procedure manual, and laboratory procedures from the national institutes of health tb research unit, as well as. Nwfhs laboratory procedures manual second edition, june 2004 chapter 5 page 3 7. Page 2 of 43 20202021 laboratory services information manual. Touch a sterile loop to a culture of the organism to be tested and pick up a visible. The waste disposal protocol in the microbiology laboratory is designed to separate the. Remove bottle cap and pour plates or dispense with a cornwall pipette, lifting each petri dish lid as you go. Microbiology a laboratory manual 11th edition 2016 pdf.
Although there are a number of comprehensive books in clinical micro biology, there remains a need for a manual that can be used in the clinical laboratory to. Mar 28, 2019 easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text. Pineapple research station k erala agricultural university, vazhakulam686 670, muvattupuzha, ernakulam, kerala. The labs are slightly selfreferential, but i believe this is necessary for students to understand the content. From the main entrance on level 2 of the john radcliffe hospital, walk down the corridor and take the central lifts to either level 6 or level 7. Keep hands and other objects away from your face, nose, eyes, ears, and mouth. Microbiology procedures guidelines 2010 edition page 6 3. On leaving the lift look for the microbiology sign on the corridor that has no wards. Basic laboratory procedures in clinical bacteriology pdf 2. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave. Throughout this manual, the student will learn the scientific method and its application. Diploma in medical laboratory technology dmlt is a professional field in allied health science.
Absolutely no food, drinks, chewing gum, or smoking is allowed in the laboratory. The following procedure and protocol manuals are provided for our microbiology laboratory and are available as an educational reference for other laboratories. Please go to the specified laboratory section of this manual for more detailed reference information on collection and submission of laboratory samples. May 03, 2011 procedures undertaken by a laboratory for the continuous evaluation of its work in order to ensure the consistency of results. In these first laboratory exercises you will be introduced to aseptic techniques, the procedures followed by. This manual has been written for a righthanded person. Furthermore, assurance must exist that testing histories are accurate and complete by having a defined system for issuance, monitoring, and reconciliation of worksheets printedused. Laboratory manual and workbook in microbiology applications to patient care 7th edition josephine a. Fdas bacteriological analytical manual bam presents the agencys preferred laboratory procedures for microbiological analyses of foods and cosmetics. The following table lists the diagnostic and reference services offered by the office of public health microbiology. Jun 28, 2020 description microbiology a laboratory manual 12th edition pdf.
The 10th edition of the manual of clinical microbiology continues to set the standard for stateofthescience laboratory practice as the most authoritative reference in the field. This laboratory does not require the use of virulent human pathogens. No food or drinks are permitted in the laboratory at any time. Guildlines for identification of aerobic bacteria, 3. Known for its thorough coverage, straightforward procedures, and minimal equipment requirements, the eleventh edition incorporates current safety protocols from governing bodies such as the epa, asm. Children under 12 years of age and pets are not allowed in laboratory. Basic information regarding preparation and procedure for sterilization.
Oct 04, 2019 this information can be found in the lab manual or related microbiology textbook. The lab clothing is not to be worn outside of the laboratory. Easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text. Page 1 of 43 20202021 laboratory services information manual laboratory services information manual 20202021 the specimen collection procedures and requirements in this manual are excerpted from current approved policies and procedures. Laboratory information system bench procedure manual. The clinical microbiology laboratory in the diagnosis of. Microbiology a laboratory manual global edition pdf. Clean the lab table before and after lab with the disinfectant solution provided 4. The specimen collection procedures and requirements in this manual are excerpted from current approved policies and procedures. West campus mcb2010c microbiology laboratory manual yasser saad, ph. Issued may, 1985 revision 21, issued may 1, 2019 this manual is the property of laboratory testing, inc. Urine culture manual prepared by qa committee issued by. Any item contaminated with bacteria or body fluids must be disposed of properly. Tmlmsh department of microbiology laboratory policy.
Proceduresguidelines for the microbiology laboratory. For example, begin your purpose statement by writing something like, in this lab experiment, 3 different types of bacteria were separated using a nutrient agar plate. Dec 15, 2017 this open lab manual is a remix of openstax microbiology, ccby 4. Bsl2criteria used when handling microorganisms that pose moderate harm, may cause disease. The first section of the manual was adapted from the openstax microbiology textbook, of which a remixed version, microbiology for allied health students, is used as the text for the course. D microbiology 3 introduction this diagnostic microbiology manual is designed to be used in conjunction with lecture textbook and other resources. Microbiology laboratory manual mount sinai hospital. Medical laboratory manual for tropical countries vol i. The lab manual was made to be used with the remixed textbook microbiology for allied health students, and the textbook has a set of instructional materials. National exposure research laboratory office of research and development cincinnati, oh office of prevention, pesticides and toxic substances office of pesticide programs ft. Competence and level of training in techniques and procedures appropria. Department of microbiology clinical laboratory manual mount.
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